Production of Acetic Indole Acid in Macrophomina phaseolina

The fungus Macrophomina phaseolina is a phytopathogen of great importance attacking various crops and causing severe losses especially in sorghum, beans, soybeans others. Since acetic indole acid (AIA) hormone that some authors associate with the pathogenic power other fungi such as Fusarium oxysporum, it was decided to perform this work detect whether M isolated from beans layers producing AIA which synthesis pathways are involved its production. analyses performed using HPLC showed production acetamide (ACM) occurs first 60 hours incubation. Another compound produced Indole (IND) released after when ACM has ceased. Similarly, can metabolize culture medium tryptophan, main precursor AIA, apparently no effect on amount synthesized.
 Conclusion: phytopathogenic produce acid, stimulates plant growth through TRP-D pathway via acetamide, mainly medium. In addition, presence detected, allows us estimate uses another little explored route TRP-I be synthesized directly or transformed into TRP. This means metabolic IAA turned off replaced by (Indole). convenient correlate release pathogenicity phaseolina.